Species:

Rat

Alternative Name:

B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10, Bcl10, Bcl-10, CARD-containing apoptotic signaling protein, CARD-containing molecule enhancing NF-kappa-B, CARD-containing proapoptotic protein, CARD-like apoptotic protein, CARMEN, caspase-recruiting domain-containing protein, cCARMEN, c-E10, c-E10bcl-10, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular homolog of vCARMEN, cellular-E10, CIPER, CLAP, hCLAP, Mammalian CARD-containing adapter molecule E10, mE10, mE10CARD containing molecule enhancing NF-kB

Assay Type:

Sandwich

unit:

pg/mL

Standard:

2000pg/mL

Sensitivity:

18.75 pg/mL

Detection Range:

31.25-2000pg/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

Research:

Tags & Cell Markers；Cell Biology；Cancer

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Bcl-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Bcl-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Bcl-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Bcl-10.You can calculate the concentration of Rat Bcl-10 in the samples by comparing the OD of the samples to the standard curve.